UV-Vis Spectrophotometer

The equipment is a double-beam UV-Vis Spectrophotometer using Shimadzu’s original LO-RAY-LIGH™ diffraction grating technology.

In addition to its high optical performance, the equipment features high resolution, low stray light, high reproducibility, and an ultra-fast scan function.

The equipment also has an easy-to-use interface on a color touch-screen display. The equipment is designed to meet the needs of both high performance and usability.



Advanced Regulatory Compliance

A Diversity of Measurement Modes

High Performance to Meet Diverse Needs

Easy to Operate, Obtain Answers Easily and Rapidly

Wavelength range190 to 1,100 nm
Spectral bandwidth1 nm (190 to 1,100 nm)
Wavelength setting0.1 nm increments
(1 nm increments when setting scanning range)
Wavelength accuracy± 0.1 nm at D2 peak 656.1 nm,
± 0.3 nm for entire range
Wavelength repeatability± 0.1 nm
Wavelength slew rateApprox. 29,000 nm/min
Wavelength scanning speed3,000 to 2 nm/min
29,000 nm/min when survey scanning
Lamp interchange wavelengthAutomatic interchange linked to wavelength.
The interchange wavelength can be set freely in the range of 295 to 364 nm (0.1 nm increments).
Stray lightLess than 0.02% at 220 nm (NaI)
Less than 0.02% at 340 nm (NaNO2)
Less than 0.5% at 198 nm (KCl)
Photometric systemDouble beam optics
Photometric rangeAbsorbance: -4 to 4 Abs
Transmittance: 0% to 400%
Photometric accuracy± 0.002 Abs at 0.5 Abs
± 0.004 Abs at 1.0 Abs
± 0.006 Abs at 2.0 Abs
(measured using NIST930D/NIST1930 or equivalent.)
Photometric repeatabilityLess than ± 0.0002 Abs at 0.5 Abs
Less than ± 0.0002 Abs at 1 Abs
Less than ± 0.001 Abs at 2 Abs
Baseline stabilityLess than 0.0003 Abs/Hr (700 nm, one hour after light source turned ON)
Baseline flatnessLess than ± 0.0006 Abs (1,100 to 190 nm, one hour after light source turned ON)
Noise levelLess than 0.00005 Abs (700 nm)
Light source20-W halogen lamp and deuterium lamp.
Built-in light source auto position adjustment.
MonochromatorLO-RAY-LIGH grade blazed holographic grating in Czerny-Turner mounting
DetectorSilicon photodiode
Sample compartmentInternal dimensions: W110 × D250 × H115 mm
Distance between light beams: 100 mm
Output deviceUSB memory (optional)
Extended memory (optional)
Data files saved in text format or UVPC format*
*Files in UVPC format can be read with the
UVProbe file viewer, which is a function of
LabSolutions™ UV-Vis, or with UVProbe software
PC compatibilityLabSolutions UV-Vis software (standard)
External control possible via USB.
Display24-bit color touch screen
Touch pen (standard included)
Touch panel protective sheet (optional)
Supported languagesJapanese, English, Chinese, Spanish (Mexico),
Portuguese (Brazil), German, French, Russian.
DimensionsW450 × D501 × H244 mm
Weight16.6 kg

Ergonomic Hardware Design
Easy-to-Use Interface Grasp the Current Status and Operating Procedures at a Glance
Navigation Tabs Improve Usability
High Performance:
Low Stray Light
High Reproducibility and Repeatability Accuracy

Power requirementsAC100,120,220,230,240 V, 50/60 Hz, 140 VA
Temperature: 15°C to 35°C
Humidity: 30% to 80%
(without condensation; 70% max. at 30°C or higher)

This is an example of the analysis of food dyes. By using
ultra-fast scan mode, the time needed for routine spectral
checks can be shortened. The 350 nm to 900 nm region can be measured at 1 nm intervals in approx. 4 seconds

Pharmaceuticals and Life Sciences
This is an example of the analysis of λDNA. Trace quantities (on the order of a few µL) can be measured by combining the instrument with Nano Stick and TrayCell®. Using TrayCell, a calibration curve for 4 µL of λDNA was obtained in the range between 25 ng/µL and 500 ng/µL.

This is an example of the analysis of colored cellophane tape.
Materials can be confirmed quantitatively by using LabSolutions UV-Vis and color measurement software.

Expanded Memory (P/N 207-23119-41)store up to 999 sets of data.

Film Holder
(P/N 204-58909) Used in transmittance measurement of thin samples such as films and filters. It holds the samples for analysis.


Long-Path Rectangular Cell Holder
(P/N 204-23118-01)
Holds two rectangular cells with an optical
path length of 10, 20, 30, 50, 70, or 100 mm.


Test Tube Holder
(P/N 207-23510-41)
Holds test tube instead of sample compartment.
Outside diameter: φ15 to 22 mm
Height: 75 to 115 mm


Multi-Cell Sample Compartment
(P/N 206-69160-41)
Holds up to six 10-mm square cells on the sample side. No temperature control capability
Number of cells:
6 on the sample side
1 on the reference side

Sipper Unit
Sipper Unit 160L  (Standard Sipper)
(P/N 206-23790-51)
Standard Sample Volume: 2.0 mL
Sipper Unit 160T (Triple-Pass Sipper)
(P/N 206-23790-52)
Standard Sample Volume: 1.5 mL
Sipper Unit 160C (Constant-Temperature Sipper)
(P/N 206-23790-53)
Standard Sample Volume: 2.5 mL
Sipper Unit 160U (Supermicro Sipper)
(P/N 206-23790-54)
Standard Sample Volume: 0.5 mL

CPS-100 Cell Positioner, Thermoelectrically Temperature Controlled
(P/N 206-29500-41)
Number of cells:
6 on the sample side (temperature-controlled)
1 on the reference side (temperature not controlled)
Temperature control range: 16°C to 60°C
Temperature display accuracy (difference from the true value): ± 0.5°C
Temperature control precision (variation of temperature): ± 0.1°C
Ambient temperature: 15°C to 35°C

TCC-100 Thermoelectrically Temperature Controlled Cell Holder
(P/N 206-29510-41)
Number of cells: One each on the sample and reference sides (temperature-controlled)
Temperature control range: 7°C to 60°C
Temperature display accuracy (difference from the true value):  ± 0.5°C
Temperature control precision (variation of temperature): ± 0.1°C

LabSolutions UV-Vis

Spectrum mode
1. Measurement modes: ABS, T%, E
2. Number of repeat scans: 1 to 99
3. Recording system: Selection between single spectrum and data overlay
4. Data storage and recall
5. Data processing:
Peak/valley detection, arithmetic operations, differentiation, smoothing, area calculation, point picking, data reading at cursor-specified point

Photometric mode
Single-wavelength measurement
1. Photometric modes: T% or Abs
2. Quantitation using K-factor method
3. Data table storage and recall functions
Multiple-wavelength measurement
4. Photometric modes: T% or ABS
5. Measurements at up to eight designated wavelengths (set in 0.1 mm increments)
6. Data calculation at up to four wavelengths (difference or ratio between two wavelengths, calculation between
three wavelengths, etc. ) is possible.

Quantitation mode
1. Measurement methods:
1-wavelength, 2-wavelength, 3-wavelength, and 1st to 4th derivative methods
2. Quantitation methods:
Automatic concentration calculation using K-factor
Automatic concentration calculation using single-point calibration curve
Multi-point calibration curve method (1st to 3rd order regression curves)
3. Measurement parameters:
Number of standards (2 to 10)
Number of repeat measurements (1 to 10 times) to obtain a mean value for quantitation.

Kinetics mode
1. Measures absorbance changes as a function of time and calculates the enzymatic activity values.
2. Measurement time: 1 to 9,999 sec/min
3. Measurement methods: 1-wavelength, 2-wavelength, multi-cell, and rate measurements

Time scan mode
1. Measures changes in measured values as a function of time.
2. Measurement mode: ABS, T%, E
3. Measurement time: 1 to 9,999 sec/min
4. Data processing functions (same as spectrum mode)

Biomethod mode
DNA/Protein Quantitation
1. Calculation of DNA/protein concentration and absorbance ratio
DNA concentration = K1 × A1 – K2 × A2
Protein concentration = K3 × A2 – K4 × A1
2. Factors and measurement wavelengths can be set freely.
3. Background correction is possible.
Quantitation of proteins
1. Quantitation methods: Lowry method, BCA method, Biuret method, CBB method (Bradford method), UV method

Shared functions
1. Automatic setting of measurement mode after instrument initialization.
It’s possible to specify standby and parameter files in the parameter setting window for each measurement mode.
2. Selection of displayed number of decimal places
Absorbance: 3 or 4 decimal places
Transmittance: 1 or 2 decimal places
3. Number of files that can be saved (built-in memory)
Measurement parameters: 100 files max.
Tabular data: 15 files max.
Curve data: 16 files max.
Validation condition: 10 files max.
Validation result: 3 files max.
4. Number of savable files (Extended memory and options)
Curve data files: 999 files max.
5. Keyboard and Barcode reader Support.
File names can be entered using the keyboard or bar code reader.
6. Wake-up function
Equipment can be started at a fixed time every day.
7. Network Connections
Multiple UV -1900i can be connected to a single printer over the network.
8. Setting of integration time (for fixed-wavelength measurement)
9. PC control
Spectrophotometer can be controlled by an external PC.
This function is also used when performing operation with the standard LabSolutions UV-Vis software provided.
*A USB cable is required.

Additional information





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